percutaneous radiologic gastrostomy tube placement without sedation

Study protocol for DICE trial: Video-assisted thoracoscopic surgery decortication versus interventional radiology guided chest tube insertion for the management of empyema

Background: Empyema is a common thoracic surgery presentation, defined as pus in the pleural space. Despite the commonality of empyema, consensus on initial management remains ambiguous. Two standard of care treatment options include inserting a chest tube (thoracostomy) and the administration of intrapleural fibrinolytics, or an initial surgical approach, surgical decortication. Due to the complexity of this pleural space infection, often repeat interventions are required after initial management in order to achieve source control and resolution of clinical symptoms. This study aims to identify the most effective initial management option for empyema.

Study design: We present a study protocol for a randomized control trial (RCT) comparing adult individuals with empyema to one of two standard of care initial management options. Participants will be randomized into either interventional radiology guided chest tube insertion with intrapleural fibrinolytics (Dornase 5 mg and Alteplase 10 mg intrapleural twice daily for three days) or video-assisted thoracoscopic surgery (VATS) decortication.

Methods: All adults with empyema meeting inclusion criteria will be invited to participate. They will be randomized into one of two intervention groups; interventional radiology guided chest tube insertion with fibrinolytics or initial VATS decortication. Each intervention will take place within 48 hours of randomization. The primary outcome will be the rate of re-intervention within 30 days. Re-intervention is defined as repeat chest tube insertion, VATS decortication, or decortication via thoracotomy. Secondary outcomes include a change in the size of empyema, length of stay, morbidity, as well as 30-day and 90-day mortality, as well as quality of life measurements.

Anticipated impact: This study is aimed at identifying the most effective initial management option for individuals with empyema

Villin Controls the Formation and Enlargement of Punctate Actin Foci in Pollen Tubes

Self-incompatibility (SI) in Papaver rhoeas triggers dramatic actin alterations in pollen. However, how actin alterations in SI pollen tubes are mechanistically achieved remains largely unexplored. Here we have used treatment with the calcium ionophore A23187 to mimic the SI-induced elevation in cytosolic Ca2+ and trigger the formation of the distinctive F-actin foci. Live-cell imaging reveals that this remodeling involves F-actin fragmentation and depolymerization, accompanied by the rapid formation of punctate actin foci and subsequent increase in their size.

We establish that actin foci are generated and enlarged from crosslinking of fragmented actin filament structures. Moreover, we show that villins associate with actin structures and are involved in this actin reorganization process. Notably, we demonstrate that Arabidopsis villin5 promotes actin depolymerization and formation of actin foci by fragmenting actin filaments, and controlling the enlargement of actin foci via bundling actin filaments. Our study thus uncovers important, novel insights about the molecular players and mechanisms involved in forming the distinctive actin foci in pollen tubes.

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geriatricsandaging

Rack for 3 x TubeSpin® Bioreactor 600 (6)

TP99013 each Ask for price

Solid screw cap for TubeSpin Bioreactor 450/600 (36)

TP87355 each Ask for price

TubeSpin Bioreactor 50 with septum (for robotic loading) (180)

TP86050 each Ask for price

MAGic Clamp™ Tube Rack, 4x500ml or 600ml conical bottles (max. 1)

H1000-MR-T600 1 PC
EUR 210.02

MAGic Clamp Tube Rack 4x500mL or 600mL conical bottles (max. 1) - EACH

MIX7170 EACH
EUR 305.1

Beaker Simax 600ml - PK10

152/600 PK10
EUR 156.6

Suspension Flasks, 600ml, Vent

25-215 5 per Sleeve, 40 Flasks/Unit
EUR 109.97
Description: Surface Area: 182.0cm2

Bottle 600ml HDPE WM + Cap

BOT3312 EACH
EUR 2.05

TC Treated Flasks, 600ml, Plug

25-210 5 per Sleeve, 40 Flasks/Unit
EUR 106.68
Description: Growth Area: 182.0cm2

TC Treated Flasks, 600ml, Vent

25-211 5 per Sleeve, 40 Flasks/Unit
EUR 110.38
Description: Growth Area: 182.0cm2

Piggy? Tube Rotator, including rotisserie for 42 x 0.5mL~0.8mL tubes, 2/pk, 50 x 1.5mL~2.0mL tubes, 2/pk, rotisserie for 14 x 5mL~7mL tubes, 2/pk, rotisserie for 14 x 10mL~15mL tubes, 2/pk, rotisserie for 6 x 50mL tubs, 1/pk

ACT-1040 each
EUR 848.4

Beaker PMP Low Form 600mL - PK12

BEA33720 PK12
EUR 270

IKA Clamp for 600mL Beaker - EACH

VIS2264 EACH
EUR 179.55

Simax Beaker Low Form 600ml - PK10

BEA10120 PK10
EUR 45.9

Duran Beaker 600ml Low Form - PK10

BEA11160 PK10
EUR 129.6

Pyrex Beaker 600ml Low Form - PK10

BEA12160 PK10
EUR 126.9

Simax Beaker Tall Form 600ml - PK10

BEA10400 PK10
EUR 60.75

Duran Beaker 600ml Tall Form - PK10

BEA11600 PK10
EUR 133.65

Pyrex Beaker 600ml Tall Form - PK10

BEA12600 PK10
EUR 132.3

Pyrex Beaker 600ml Heavy Duty - PK6

BEA12840 PK6
EUR 118.8

Academy Low Form Beaker 600ml - PK6

BEA34100 PK6
EUR 16.2

Azlon Beaker PP 600ml Stacking - PK5

BEA5132 PK5
EUR 32.4

Academy Heavy Duty Beaker 600ml - PK6

BEA0126 PK6
EUR 55.49

Solid liq Extrac Assembly 600ml - EACH

Q600RDSX EACH
EUR 1031.4

Basin Evaporating FB Spout 600ml - PK10

BAS2026 PK10
EUR 294.3

IKA Double-Walled Beaker 600mL - EACH

VIS2262 EACH
EUR 936.9

Centrifuge Tubes S 2420 30ml Qfit - PK10

QBC24C24R0 PK10
EUR 270

FA-6x250 rotor for 6 x 250mL tubes 5910 R/5920 R - EACH

E5895175007 EACH
EUR 4862.7

Insert For 10 Beakers 250-600ml - EACH

WAS6076 EACH
EUR 348.11

Azlon Beaker 600ml PP Low Form - PK5

BEA5034 PK5
EUR 37.8

Nalgene Beaker 600ml PP Low Form - PK4

BEA3352 PK4
EUR 51.3

Beaker tall form heavy duty 600mL - PK10

BEA3024 PK10
EUR 139.05

SLS Select Trigger Spray Bottle 600ml - EACH

CLE9010 EACH
EUR 9.17

Optional generator 14mm x 130mm saw tooth, for 50ml tubes up to 250ml vessels

BCM1236 1 pcs, 1 UNIT
EUR 1407.54

Optional generator 14mm x 130mm saw tooth, for 50ml tubes up to 250ml vessels

D1000-M14 ea
EUR 1290

15PL 15mL Rack for 15mL Tubes - EACH

HS23215 EACH
EUR 49.55

Adapter pack, for 5ml and 7ml (12-13mm) tubes in 15ml rotors, 8/pk.

C3100-ADP 1 PC
EUR 121.95

ABgene 0.65mL Blank Tubes, 10 racks of 96 tubes/case

MAAB-1256 each
EUR 455.08

Falcon Cushion For 175ml And 225ml Tubes - PK8

352090 PK8
EUR 97.2

ADAPTERS FOR 10/15ML TUBES IN 50ML ROTOR,6/CS

480140 6/pk
EUR 144
Description: Lab Equipment; General Purpose Centrifuges (affliated brand)

ADAPTERS FOR 5/7/10ML TUBES IN 15ML ROTOR,6/CS

480142 6/pk
EUR 136.8
Description: Lab Equipment; General Purpose Centrifuges (affliated brand)

Magnetic Stand for 4 Tubes (15mL, 50mL)

MB-01 1EA
EUR 267.5
Description: The Magnetic Stand is the indispensable tool for customers to use magnetic bead products.The Magnetic Stand contains magnets with strong magnetic attraction, which can be separated in a centrifuge tube to achieve rapid separation and purification of substances such as cells, proteins, or nucleic acids, and has a wide range of applications.

0.1ML PCR STRIP TUBES AND CAPS (0.07ML WORKING VOLUME)4 TUBES/4 CAPS PER STRIP, 250 STRIP TUBES AND STRIP CAPS PER UNIT

PCR-0104-C 250/pk
EUR 1244.4
Description: PCR Plates & Tubes; PCR Tubes - Axygen

BEAKER,600ML,PLASTIC,LOW FORM,PP,6/6

1000P-600 6/pk
EUR 67.2
Description: Reusable Plastics; Resuable Beakers

Nunc Rack for 6 x 200ml Tubes - EACH

374179 EACH
EUR 129.6

StrepMan Magnet for 15ml and 50ml tubes

IB6-5650-065 each
EUR 420.74

12 Well Tube Rack 5/15mL Tubes - PK2

E0030119827 PK2
EUR 59.4

Adapter for 4-15ml Tubes - EACH

GBA1763 EACH
EUR 202.5

Adapter for 85ml round bucket for use with standard Vacu- tainer and Falcon tubes 9-15ml 2 pcs. - PK2

E5702724009 PK2
EUR 217.35

Adapter for 85ml round bucket for use with standard Vacu- tainer and Falcon tubes 4-10ml 2 pcs. - PK2

E5702735000 PK2
EUR 217.35

Basin Pyrex 600ml Flat Form Curved Sides - PK10

BAS15100 PK10
EUR 375.3

Kimble Tall Form Beaker With Spout 600ml - PK10

BEA3456 PK10
EUR 83.72

Adapter 50mL tubes Centriprep 30mL - PK2

E5910764006 PK2
EUR 359.1

Aluminum housed mantle for griffin beaker 600ml, 325W, 115V

100BTM610 each
EUR 363

Aluminum housed mantle for griffin beaker 600ml, 325W, 230V

100BTM611 each
EUR 379

Fabric mantle for 600ml griffin beaker, 325W, 115V

100AO610 each
EUR 394

Fabric mantle for 600ml griffin beaker, 325W, 230V

100AO611 each
EUR 412

Test Tube rack for 41 x 15mL tubes - EACH

BAT3070 EACH
EUR 163.35

Test Tube rack for 15 x 50mL tubes - EACH

BAT3072 EACH
EUR 163.35

Test Tube rack for 76 x 15mL tubes - EACH

BAT3074 EACH
EUR 325.35

Test Tube rack for 30 x 50mL tubes - EACH

BAT3076 EACH
EUR 325.35

DURAN Beaker High Form Without Spout 600mL - PK10

BOT0550 PK10
EUR 126.9

Block For 24 X 5-7mL Tubes (12mm) - EACH

SHA2104 EACH
EUR 780.3

ADAPTERS FOR 5/7ML TUBES IN 50ML ROTOR,6/CS

480141 6/pk
EUR 144
Description: Lab Equipment; General Purpose Centrifuges (affliated brand)

50ml Plug-Seal Centrifuge Tubes with Rack, 25 tubes/rack

10-0501 -
EUR 12.5

Adaptor For 7 X 15ml Tubes

CEN0634 EACH
EUR 140.22

Mini Cooler 0c 12 x 0.5-2ml Tubes

FRE0003 EACH
EUR 380.16

Universal Rack For 12 x 15-50mL Tubes - EACH

RAC2214 EACH
EUR 113.4

250mL centrifuge tube PP (59.8 X 160mm), with screw-cap, 14 bags of 5 tubes

C1250 70
EUR 234

Adapter pack for C5000-6H and C5000-8, for 5ml and 7ml (12-13mm) tubes, 8/pk.

C5000-ADP 1 each
EUR 118.24

Reducing Adapter for 15mL/5mL tubes, 25/pk

C4015 25/pack
EUR 47.88
Description: Adapter for SureStrain Premium Cell Strainers

Conical Tubes 50mL - PK48

E0030122267 PK48
EUR 58.05

Adaptor For 50ml Tubes - PK8

E5804737008 PK8
EUR 124.2

Adapter for 5/15mL tubes - PK2

E5910702000 PK2
EUR 301.05

Adapter for 15 x 4-8mL tubes - PK2

E5910703007 PK2
EUR 301.05

Adapter for 50mL tubes - PK2

E5910712006 PK2
EUR 279.45

Insert for 2 x 10ml Tubes - EACH

CEN0524 EACH
EUR 153.9

Insert for 15ml Tubes - EACH

CEN0642 EACH
EUR 153.9

Tubes 50ml Conical HD - PK12

X13758 PK12
EUR 233.55

Mini Cooler -20c 32 x 0.5-2ml Tubes

FRE0006 EACH
EUR 380.16

CryoBox 81 Places for 3-4-5mL Tubes - Red - PK4

CRY1132 PK4
EUR 44.55

Optional generator, 10mm x 115mm saw tooth, for 15ml & 50ml tubes

D1000-M10 ea
EUR 390

Culture Tube, 14mL, 17 x 100mm, PP, w/ attached 2-position screw-cap, printed graduations, sterile, 20 bags of 25 tubes, 500/case

T8830 500/pack
EUR 207.58
Description: Culture Tube 14mL

Culture Tube, 14mL, 17 x 100mm, PP, w/ attached 2-position screw-cap, printed graduations, sterile, 10 foam racks of 50 tubes, 500/case

T8832 500/pack
EUR 255.73
Description: Culture Tube 14mL

Culture Tube, 14mL, 17 x 100mm, PS, w/ attached 2-position screw-cap, printed graduations, sterile, 20 bags of 25 tubes, 500/case

T8840 500
EUR 225

Culture Tube, 14mL, 17 x 100mm, PS, w/ attached 2-position screw-cap, printed graduations, sterile, 10 foam racks of 50 tubes, 500/case

T8842 500
EUR 259

Adapter Pack for 5mL Tubes in 15mL Cavity - PK4

CEN2063 PK4
EUR 76.95

25ml PP (27x77mm), with screw cap, sterile, 8 bags of 25 tubes, 200/pack

C2625-B 200/pack
EUR 101.2

CoolRack 50 ml 4 x 50ml tubes - EACH

432062 EACH
EUR 432

Conical Tubes 15mL - PK200

E0030122208 PK200
EUR 89.1

Conical Tubes 50mL - PK200

E0030122232 PK200
EUR 106.65

Conical Tubes 15mL - PK100

E0030122259 PK100
EUR 102.6

Rotor Angle 6 x 85ml Tubes - EACH

CEN0641 EACH
EUR 2204.55

Mini Cooler 0c 12 x 0.5-2ml Tubes - EACH

FRE0001 EACH
EUR 305.1

Mini Cooler 0c 32 x 0.5-2ml Tubes - EACH

FRE0002 EACH
EUR 488.7

Adapter for 4 x 15/50ml tubes, pack of 2

IPD9600-50T each
EUR 1946.7

Rack For 50ml Tubes 12 Place - EACH

SHA4452 EACH
EUR 461.7

Disk modul for RS-RD 20, 8 x 50ml tubes

RSE-RD15 8 x 50ml tubes
EUR 99

Optional generator, 10mm x 115mm saw tooth, for 15ml and 50ml tubes

BCM1235 1 pcs, 1 UNIT
EUR 517.18

Adapter for 100ml rectangular bucket for use with 50-75ml tubes 2 pcs. - PK2

E5804760000 PK2
EUR 172.8

Adapter for 250ml rectangular bucket for use with 180-250ml tubes 2 pcs. - PK2

E5810770009 PK2
EUR 228.15

CryoBox 81 Places for 3-4-5mL Tubes - Green - PK4

CRY1134 PK4
EUR 47.25

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 85.2

Rotor Angle + lid 6 x 50ml Tubes - EACH

CEN0060 EACH
EUR 1039.5

Adapter D26mm 30ml Tubes - PK2

CEN2640 PK2
EUR 140.4

Adapter D18mm 16ml Tubes - PK2

CEN2642 PK2
EUR 140.4

Adapter D17mm 15ml Tubes - PK2

CEN2644 PK2
EUR 140.4

Palintest 10ml Tubes Pk5 - PK5

WAT8034 PK5
EUR 52.65

Mini Cooler -20c 12 x 0.5-2ml Tubes - EACH

FRE0004 EACH
EUR 252.45

Mini Cooler -20c 32 x 0.5-2ml Tubes - EACH

FRE0005 EACH
EUR 403.65

A method for percutaneous radiologic gastrostomy tube placement without sedation as a bridge to lung transplantation

Gastrostomy tube placement is an appropriate option for long-term nutritional support for patients who cannot tolerate oral intake. Common indications for a gastrostomy tube include head and neck tumors and neurological disorders. Several methods for gastrostomy tube insertion exist (eg, surgical, endoscopic, and radiologic) that require sedation or general anesthesia, which can pose risks of cardiopulmonary compromise and postsurgical pulmonary complications. Unlike other methods, our practice uses a percutaneous balloon-assisted gastrostomy tube insertion method for which we can perform without sedation.

We report a case of a percutaneous radiologic gastrostomy procedure for a patient with end stage lung disease as a bridge to lung transplantation, who is not a candidate for sedation and is high-risk for general anesthesia. Through enteral feeds administered through the successfully placed gastrostomy tube, the patient showed steady improvement in weight gain over the course of several months before approval for listing by the lung transplant selection committee. Our case highlights how gastrostomy tube placement can be safely performed in patients who are not sedation candidates using the minimally invasive balloon-assisted gastrostomy tube insertion method and local anesthetic.

Passive Release Technique Produces the Most Accurate Endotracheal Tube Cuff Pressure Than Manual Palpation and Minimum Occlusive Volume Technique in the Absence of Manometer

Objective: This study aimed to compare the accuracy of the endotracheal tube (ETT) cuff pressure of the manual palpation (MP), passive release (PR), and minimum occlusive volume (MOV) techniques.

Methods: This study is a true experiment with simple randomisation. The subjects of this study were 105 patients divided into 3 groups: MP group (n=35), PR group (n=35), and MOV group (n=35). After intubation, ETT cuff inflation was performed using 3 different techniques. The ETT cuff pressure was recorded using a manometer. The data were analysed using the chi-square test, Kruskal-Wallis test, and Mann-Whitney test in the SPSS 20 software.

Results: The mean ETT cuff pressure was 60.2±28.8 cmH2O in the MP group, 30.4±5.5 cmH2O in the PR group, and 25.8±9.6 cmH2O in the MOV group (p=0.000). The PR group had the highest pressure accuracy (77%) (p=0.000).

Conclusion: The PR technique had the highest accuracy and can be used as an alternative ETT cuff inflation technique in the absence of a manometer.

Non-contact detection of thiodiglycol vapors and associated degradation products using atmospheric flow tube mass spectrometry

Thiodiglycol (TDG) is a synthetic precursor and an environmental degradation product of sulfur mustard (HD). Consequently, its presence can be indicative of illicit preparation or historical presence of chemical weapons, but its lower toxicity lends itself to use as an HD simulant for testing and method development. Detection of TDG vapor often proves elusive with existing techniques exhibiting undesirably high detection limits in the gas phase (>ppm). Moreover, traditional approaches to detecting TDG vapor rely upon non-specific approaches that do not provide the certainty afforded by mass spectrometry.

Using atmospheric flow tube mass spectrometry (AFT-MS), which has previously demonstrated the capacity to detect parts-per-quadrillion levels of vapor, we evaluate the capacity of this approach for non-contact residue analysis based upon TDG vapor sampling and nitrate clustering chemistry. Furthermore, we discuss challenges with ambient vapor detection using the AFT-MS system and associated observations related to TDG degradation into 2,2′-sulfonyldiglycol from exposure to ambient conditions with vapor detection being possible even after 7-weeks of sample aging.

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